Electricity Production and Phenol Removal of Winery Wastewater by Constructed Wetland – Microbial Fuel Cell Integrated With Ethanol Tolerant Yeast

Abstract

Winery wastewater is one of the most concerning wastewater because it contains a high concentration of ethanol and phenolic compound that can inhibit the wastewater treatment potential of microorganisms. In this study, the ethanol tolerant yeast with laccase activity was integrated with constructed wetland -microbial fuel cell (CW-MFC) to remove ethanol contamination wastewater treatment limitation and simultaneously generate electrical energy. For the experiment, ethanol tolerant yeast with laccase activity was selected and grown in the CW-MFC filled with winery wastewater. The wastewater treatment (chemical oxygen demand (COD) and phenol removal) potential and electrochemical properties were monitored. The results indicated that the maximal laccase activity of 158.12±0.52 U/mL was gained from the yeast strain ET-KK. The maximal current density and power density of 139.17±1.44 mA/m2 and 38.74±0.80 mW/m2 were generated where the maximal COD and phenol removal of 79.14±0.92% and 85.04±0.07% were obtained. This study used the CW-MFC integrated with laccase-producing ethanol tolerant yeast for winery wastewater treatment and electricity generation.