Ribosomal RNA 2′- <i>O</i> -methylations regulate translation by impacting ribosome dynamics

Abstract

SignificanceThe presence of RNA chemical modifications has long been known, but their precise molecular consequences remain unknown. 2'-<i>O</i>-methylation is an abundant modification that exists in RNA in all domains of life. Ribosomal RNA (rRNA) represents a functionally important RNA that is heavily modified by 2'-<i>O</i>-methylations. Although abundant at functionally important regions of the rRNA, the contribution of 2'-<i>O</i>-methylations to ribosome activities is unknown. By establishing a method to disturb rRNA 2'-<i>O</i>-methylation patterns, we show that rRNA 2'-<i>O</i>-methylations affect the function and fidelity of the ribosome and change the balance between different ribosome conformational states. Our work links 2'-<i>O</i>-methylation to ribosome dynamics and defines a set of critical rRNA 2'-<i>O</i>-methylations required for ribosome biogenesis and others that are dispensable.