Protein blotting

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Harry Towbin, T. Staehelin, J. Gordon · Proceedings of the National Academy of Sciences · 1979 · 53.8K citations · Full text

Life Sciences, Biochemistry, Molecular Biology +13

Detection of specific sequences among DNA fragments separated by gel electrophoresis

E.M. Southern · Journal of Molecular Biology · 1975 · 32.8K citations

Life Sciences, Molecular Biology, Molecular Genetics +12

“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A

W. Neal Burnette · Analytical Biochemistry · 1981 · 8.5K citations

Life Sciences, Biochemistry, Molecular Biology +15

Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphenylglycoluril

Pamela J. Fraker, John C. Speck · Biochemical and Biophysical Research Communications · 1978 · 4.6K citations

Life Sciences, Biochemistry, Cell Biology +8

Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polycrylamide to nitrocellulose

Jan Kyhse‐Andersen · Journal of Biochemical and Biophysical Methods · 1984 · 2.5K citations

Life Sciences, Biochemistry, Molecular Biology +11