“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A

W. Neal Burnette

Analytical Biochemistry

BiochemistryNatural SciencesBioanalysisMolecular BiologyProtein A +8

The isolation of hybrid cell lines producing monoclonal antibodies against the p15(E) protein of ecotropic murine leukemia viruses

Robert C. Nowinski, Mark E. Lostrom, Milton R. Tam, +2

Virology

Molecular VirologyPathogenesisImmunologyHybrid Cell LinesVirology +6

Pertussis toxin has eukaryotic-like carbohydrate recognition domains.

Kapo Saukkonen, W. Neal Burnette, V L Mar, +2

Proceedings of the National Academy of Sciences

Microbial ToxinPertussis ToxinToxinologyBordetella PertussisMedicine +9

Monoclonal antibodies against murine leukemia viruses: Identification of six antigenic determinants on the p15(E) and gp70 envelope proteins

Mark E. Lostrom, Mary R. Stone, Milton R. Tam, +3

Virology

Virus StructureMolecular VirologyMurine Leukemia VirusesImmunologyAntiviral Response +7

Pertussis Toxin S1 Mutant with Reduced Enzyme Activity and a Conserved Protective Epitope

W. Neal Burnette, Witold Cieplak, V L Mar, +3

Science

ToxinologyImmunologyImmunodominancePertussis ToxinVaccine Target +14

Role of carbohydrate recognition domains of pertussis toxin in adherence of Bordetella pertussis to human macrophages

J van't Wout, W. Neal Burnette, V L Mar, +3

Infection and Immunity

Microbial ToxinBordetella PertussisMedicineVirulence FactorPathogenesis +10

Site-specific mutagenesis of the catalytic subunit of cholera toxin: substituting lysine for arginine 7 causes loss of activity

W. Neal Burnette, V L Mar, B W Platler, +5

Infection and Immunity

Microbial ToxinArginine 7BiochemistryToxinologyMedicine +13

Direct Expression of Bordetelia pertussis Toxin Subunits to High Levels in Escherichia coli

W. Neal Burnette, V L Mar, Witold Cieplak, +6

Nature Biotechnology

Microbial ToxinDirect ExpressionToxinologyVirulence FactorPathogenesis +8

Identification of a region in the S1 subunit of pertussis toxin that is required for enzymatic activity and that contributes to the formation of a neutralizing antigenic determinant.

Witold Cieplak, W. Neal Burnette, V L Mar, +5

Proceedings of the National Academy of Sciences

Homology BoxesProtein ExpressionToxinologyHomology BoxMedicine +11

Construction and characterization of recombinant Vibrio cholerae strains producing inactive cholera toxin analogs

Claudia C. Häse, L S Thai, M Boesman-Finkelstein, +6

Infection and Immunity

ToxinologyMolecular BiologyProtein SynthesisBiosynthesisCatalytic A Subunit +14