Addendum: Transcriptomics and proteomics reveal two waves of translational repression during the maturation of malaria parasite sporozoites

Abstract

In the original version of the Article, an inadvertent operator error resulted in our processing of the RNA-seq data to not account for the correct strandedness of the mapped reads (e.g., a single toggle was not changed from the default). Here with the key contributions of our bioinformatician colleagues (now added as co-authors), we have thus reanalyzed these RNA-seq data (described below) and we provide the downstream interpretations based upon them in this Addendum. Importantly, upon a comparison of the rank abundance of the sense and antisense mapped reads, we observed strong correlations for both P. falciparum and P. yoelii in both stages of sporozoite development (Supplementary Fig. Perhaps due to this, the major conclusions of the original Article still are valid and do not require modification. However, the new analyses required modification of the composition of some gene lists. In addition, we have now also identified and accounted for run-in transcription through the application of a Transcript Integrity Number (TIN) metric that is applied to all transcripts to make these datasets more robust 1 . We provide those corrections to the gene lists and relevant figures and Supplementary Files here.