Cytokinin Perception in Ancient Plants beyond Angiospermae

Abstract

Cytokinins (CKs) control many plant developmental processes and responses to environmental cues. Although the CK signaling is well understood, we are only beginning to decipher its evolution. Here, we investigated the CK perception apparatus in early-divergent plant species such as bryophyte <i>Physcomitrium patens</i>, lycophyte <i>Selaginella moellendorffii</i>, and gymnosperm <i>Picea abies</i>. Of the eight CHASE-domain containing histidine kinases (CHKs) examined, two CHKs, PpCHK3 and PpCHK4, did not bind CKs. All other CHK receptors showed high-affinity CK binding (<i>K</i>_D of nM range), with a strong preference for isopentenyladenine over other CK nucleobases in the moss and for <i>trans</i>-zeatin over <i>cis</i>-zeatin in the gymnosperm. The pH dependences of CK binding for these six CHKs showed a wide range, which may indicate different subcellular localization of these receptors at either the plasma- or endoplasmic reticulum membrane. Thus, the properties of the whole CK perception apparatuses in early-divergent lineages were demonstrated. Data show that during land plant evolution there was a diversification of the ligand specificity of various CHKs, in particular, the rise in preference for <i>trans</i>-zeatin over <i>cis</i>-zeatin, which indicates a steadily increasing specialization of receptors to various CKs. Finally, this distinct preference of individual receptors to different CK versions culminated in vascular plants, especially angiosperms.