Comprehensive Analysis of Epigenetic Modifications and Immune-Cell Infiltration In Tissues from Patients With Systemic Lupus Erythematosus

Abstract

<b>Aim:</b> To explore potential abnormal epigenetic modifications and immune-cell infiltration in tissues from systemic lupus erythematosus (SLE) patients. <b>Materials & methods:</b> To utilize bioinformatics analysis and 'wet lab' methods to identify and verify differentially expressed genes in multiple targeted organs in SLE. <b>Results:</b> Seven key genes, <i>IFI44</i>, <i>IFI44L</i>, <i>IFIT1</i>, <i>IFIT3</i>, <i>PLSCR1</i>, <i>RSAD2</i> and <i>OAS2</i>, which are regulated by epigenetics and may be involved in the pathogenesis of SLE, are identified by combined long noncoding RNA-miRNA-mRNA network analysis and DNA methylation analysis. The results of quantitative reverse transcription PCR, immunohistochemistry and DNA methylation analysis confirmed the potential of these genes as biomarkers. <b>Conclusion:</b> This study reveals the potential mechanisms in SLE from epigenetic modifications and immune-cell infiltration, providing diagnostic biomarkers and therapeutic targets for SLE.