Biochemical Journal · 1991 · 41 citations · 27 references
Life SciencesBiochemistryMolecular BiologyGene ExpressionProtein EngineeringSynthetic BiologyEnzyme ExpressionFatty Acid MetabolismLipid BiosynthesisLipid MetabolismProtein Expression EngineeringProtein SynthesisBacterial Protein SynthesisEscherichia Coli Expression SystemsFatty Acid BindingFatty Acid-responsive Gene Expression
A gene coding for rat liver fatty-acid-binding protein (FABP) has been constructed by the single-step ligation of ten synthetic oligonucleotides. The gene has been cloned into the bacterial expression vector pKK223-3. Induction of protein synthesis from this gene results in over expression of an FABP that is indistinguishable in its structure and binding properties with that isolated from rat liver.
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