A Robust Platform for Unnatural Amino Acid Mutagenesis in E. coli Using the Bacterial Tryptophanyl-tRNA synthetase/tRNA pair

One-step inactivation of chromosomal genes in <i>Escherichia coli</i> K-12 using PCR products

Kirill A. Datsenko, Barry L. Wanner · Proceedings of the National Academy of Sciences · 2000 · 15.2K citations · Full text

Life Sciences, Genetics, Molecular Genetics +17

Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes

F. William Studier, Barbara A. Moffatt · Journal of Molecular Biology · 1986 · 6.4K citations

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Programming cells by multiplex genome engineering and accelerated evolution

Harris H. Wang, Farren J. Isaacs, Peter A. Carr et al. · Nature · 2009 · 1.6K citations

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An Enhanced System for Unnatural Amino Acid Mutagenesis in E. coli

Travis S. Young, Insha Ahmad, Jun Yin et al. · Journal of Molecular Biology · 2009 · 649 citations

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Molecular characterization of vulnibactin biosynthesis in Vibrio vulnificus indicates the existence of an alternative siderophore

Wenzhi Tan, Vivek Verma, Kwangjoon Jeong et al. · Frontiers in Microbiology · 2014 · 637 citations · Full text

Life Sciences, Microbiology, Molecular Biology +12