The Effect of Substrate Temperature on the Evaporative Behaviour and Desiccation Patterns of Foetal Bovine Serum Drops

Abstract

The drying of bio-fluid drops results in the formation of complex patterns, which are morphologically and topographically affected by environmental conditions including temperature. We examine the effect of substrate temperatures between 20 °C and 40 °C, on the evaporative dynamics and dried deposits of foetal bovine serum (FBS) drops. The deposits consist of four zones: a peripheral protein ring, a zone of protein structures, a protein gel, and a central crystalline zone. We investigate the link between the evaporative behaviour, final deposit volume, and cracking. Drops dried at higher substrate temperatures in the range of 20 °C to 35 °C produce deposits of lower final volume. We attribute this to a lower water content and a more brittle gel in the deposits formed at higher temperatures. However, the average deposit volume is higher for drops dried at 40 °C compared to drops dried at 35 °C, indicating protein denaturation. Focusing on the protein ring, we show that the ring volume decreases with increasing temperature from 20 °C to 35 °C, whereas the number of cracks increases due to faster water evaporation. Interestingly, for deposits of drops dried at 40 °C, the ring volume increases, but the number of cracks also increases, suggesting an interplay between water evaporation and increasing strain in the deposits due to protein denaturation.