BMC Research Notes · 2021 · 15 citations · 16 references
EngineeringBioengineeringBiomedical EngineeringBiomaterialsCartilage RepairTissue EngineeringCartilage BiologyCartilage RegenerationCartilage Repair MaterialsCartilage Tissue EngineeringGelsHydrogelsAlginate-based BiomaterialsAlginate-based GelsCartilage Regeneration BiomaterialsEnzymatic Cross-linkingGelationGelation TimeHyaline Cartilage EngineeringHydrogel Characterization
Abstract Objective We aimed to detect the effect of a couple of parameters including Alg, H 2 O 2 , and HRP concentrations on the gelation time of Alg-based hydrogels using an enzymatic cross-linked procedure. Results NMR, UV–Vis, and ATR-FTIR analyses confirmed the conjugation of Ph to the Alg backbone. Data showed gelation time was delayed with the increase and reduction of H 2 O 2 and HRP, respectively. We noted that hydrogel consisted of 1.2% (w/v) Alg, 5 U HRP, and 100 mM H 2 O 2 yielded an appropriate gelation time with appropriate mechanical properties. The addition of 0.5% (v/v) Col developed hydrogel increased the gelation time. The data showed that Alg, HRP, and H 2 O 2 with the ratio of 1:0.54:0.54 had proper physicochemical features for cartilage engineering.
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