Life SciencesBiotechnologyGeneticsGene ExpressionGene RegulationSynthetic BiologyGene Expression RegulationGene Regulation In MicrobesPromoter EngineeringPromoter RegulationPromoter SelectionYeast GeneticsAlcohol ProductionEscherichia Coli Expression SystemsExpression Host EngineeringGolden Gate AssemblyShuttle VectorsYeast Expression VectorsYeast PromotersYeast Strain Engineering
. The utility of shuttle vectors was demonstrated for rapid phenotyping of a multigene pathway for cinnamyl alcohol production. Taken together, our work opens a new avenue for the future development of broad host expression systems between prokaryotic and eukaryotic hosts.
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Patrick J. Westfall, Douglas J. Pitera, Jacob R. Lenihan et al. · Proceedings of the National Academy of Sciences · 2012 · 725 citations · Full text