Preparation of a Porphyrin Metal–Organic Framework with Desirable Photodynamic Antimicrobial Activity for Sustainable Plant Disease Management

Abstract

Considering the severity of plant pathogen resistance toward commonly used agricultural microbicides, as well as the potential threats of agrichemicals to the eco-environment, there is a pressing need for antimicrobial approaches that are capable of inactivating pathogens efficiently without the risk of inducing resistances and harm. In this work, a porphyrin metal-organic framework (MOF) nanocomposite was constructed by incorporating 5,10,15,20-tetrakis(1-methyl-4-pyridinio)porphyrin tetra(<i>p</i>-toluenesulfonate) (TMPyP) as a photosensitizer (PS) in the cage of a variant MOF (HKUST-1) to efficiently produce singlet oxygen (¹O₂) to inactivate plant pathogens under light irradiation. The results showed that the prepared PS@MOF had a loading rate of PS about 12% (w/w) and excellent and broad-spectrum photodynamic antimicrobial activity in vitro against three plant pathogenic fungi and two pathogenic bacteria. Moreover, PS@MOF showed outstanding control efficacy against <i>Sclerotinia sclerotiorum</i> on cucumber in the pot experiment. <i>Allium cepa</i> chromosome aberration assays and safety evaluation on cucumber and Chinese cabbage indicated that PS@MOF had no genotoxicity and was safe to plants. Thus, porphyrin MOF demonstrated a great potential as an alternative and efficient new microbicide for sustainable plant disease management.