Antioxidant and cytotoxic activities of the ethyl acetate extract of Sphagneticola trilobata (L.) J.F. Pruski on MCF-7 breast cancer cell

Abstract

<i>Sphagneticola trilobata</i> (L.) J.F. Pruski is the perennial herb distributed at tropical temperature. In this study, the antioxidant and anticancer properties of the ethyl acetate extract from <i>S. trilobata</i> leaves were investigated against MCF-7 breast cancer cells. The antioxidant and anticancer activities were assessed by 1,1-diphenyl-2-picrylhydrazyl free radical scavenging and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay methods, respectively. The extract demonstrated DPPH free radical scavenging effect with IC₅₀ value equaling to 127.43 μg/mL. The cytotoxic study was conducted on the concentration range of 1-200 μg/mL, and the results exhibited the strongest inhibitory activity against MCF-7 with the LC₅₀ value of 58.143 μg/mL. The cytotoxic activity of the extract was supported by the induction of apoptosis cell which possessed the apoptosis percentage of 78.80%. Thus, the cheap herbal drug treatment might highly be recommended to treat effectively breast cancers as an ideal choice or combinational therapy.