Concepedia

A sensitive and specific method for microRNA detection and <i>in situ</i> imaging based on a CRISPR–Cas9 modified catalytic hairpin assembly

Yang Liu, Shihong Li, Likun Zhang, Qian Zhao, Nuo Li, Yuxin Wu

RSC Advances · 2020 · 29 citations · 18 references

DOIFull text

Open access

Concepts

Abstract

We report here a method for the molecular detection of miRNAs in exosomes and imaging in living cells based on CRISPR–Cas9 and catalytic hairpin assembly.

References

18

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

Janice S. Chen, Enbo Ma, Lucas B. Harrington et al. · Science · 2018 · 4.4K citations

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Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components

Keith Pardee, Alexander A. Green, Melissa K. Takahashi et al. · Cell · 2016 · 1.5K citations · Full text

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Rolling Circular Amplification (RCA)-Assisted CRISPR/Cas9 Cleavage (RACE) for Highly Specific Detection of Multiple Extracellular Vesicle MicroRNAs

Ruixuan Wang, Xianxian Zhao, Xiaopei Qiu et al. · Analytical Chemistry · 2019 · 320 citations

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CASFISH: CRISPR/Cas9-mediated in situ labeling of genomic loci in fixed cells

Wulan Deng, Xinghua Shi, Robert Tjian et al. · Proceedings of the National Academy of Sciences · 2015 · 288 citations · Full text

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Rapid and sensitive exosome detection with CRISPR/Cas12a

Xianxian Zhao, Wenqing Zhang, Xiaopei Qiu et al. · Analytical and Bioanalytical Chemistry · 2020 · 188 citations

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