Proceedings of the National Academy of Sciences · 2020 · 51 citations · 59 references
Life SciencesGeneticsNeuroscienceDevelopmental NeuroscienceMolecular NeuroscienceTranscriptomicsNeural DevelopmentNeural Stem Cell BiologyNeural Stem CellsNeurogenesisSingle-cell Rna Sequencing AnalysisSingle-cell TranscriptomicsCell Fate MappingCell Lineage TracingCre-loxp Inducible SystemsDendritic MorphogenesisNeural Fate SpecificationNeural Lineage SpecificationRna Sequencing (Rna-seq)Single-cell Rna Sequencing
Significance The tamoxifen (TAM)-inducible CreER/LoxP system is one of the most widely used genetic tools that have enabled precise gene manipulation in distinct cell subpopulations at any specific time point and labeling of any cell types for genetic fate mapping. We report that administration of TAM during early stages of cortical neurogenesis inhibits neural progenitor cell proliferation and impaired cortical neurogenesis, dendritic morphogenesis, synaptic density, and gliogenesis in postnatal offspring. By employing single-cell RNA sequencing analysis combined with in vivo and in vitro assays, we found TAM could exert these drastic effects through dysregulating the Wnt-Dmrta2 signaling pathway. This study shows that care must be taken when using the TAM-induced CreER/LoxP system for neural lineage tracing and genetic manipulation studies.
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Spatial reconstruction of single-cell gene expression data
Rahul Satija, Jeffrey A. Farrell, David Gennert et al. · Nature Biotechnology · 2015 · 7.7K citations · Full text
Michael J. Hendzel, Wei Yi, Michael A. Mancini et al. · Chromosoma · 1997 · 1.9K citations