IOP Conference Series Materials Science and Engineering · 2020 · 10 citations · 24 references
Natural SciencesChemistryPharmaceutical AnalysisAnalytical ChemistrySpectroscopic AnalysisSpectroscopyBioanalytical Method ValidationColor AnalysisEnvironmental AnalysisInstrumental AnalysisPhysicochemical CharacterizationSpectroscopic Analysis Of PharmaceuticalsAnalytical Method OptimizationCalibration Curve AnalysisCalibration Curve FittingCloud PointCloud Point ExtractionConcentrationUltraviolet-visible DetectionUv-visible Quantitative Analysis
Abstract In this study, new spectrophotometric methods development for evaluation of ciprofloxacin are described. The first method including conversion of ciprofloxacin to colored complex with Fe (II) in the alkaline medium. The colored product has a yellow color with absorbance at λ max 450 nm. Between the concentration range (2-90 μg.mL −1 ), the Beers law is obeyed with correlation coefficient (R 2 ) as 0.9996, limit of detection as 0.365 μg.mL −1 , limit of quantification as 1.189 μg.mL −1 . Secondly, cloud point extraction was used to determine of a trace amount of the colored product in the first method followed by measuring with a UV-Vis spectrophotometer. The linearity of calibration curve was above the range of (1-50μg.mL −1 ), the correlation coefficient (R 2 ) was 0.9995. The Limit of detection (LOD) and Limit of quantification (LOQ) were found to be 0.129 and 0.387 μg.mL 1 respectively. This method was successfully employed for ciprofloxacin detection in the several pharmaceutical samples by Rec. Was rang between (98-102.8).
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