RNA interference therapeutics targeting angiotensinogen ameliorate preeclamptic phenotype in rodent models

Abstract

The RUPP procedure is a well-established model for studying the link between placental ischemia and hypertension in the pregnant rat and has been described in detail previously.( 1) First timed pregnant Sprague-Dawley rats purchased from Harlan Sprague Dawley Inc. (Indianapolis, IN) were used in this study.Twenty-eight pregnant rats were randomly assigned to either the NP, NP rAgt-targeting siRNA, RUPP or the RUPP rAgt-targeting siRNA group.On day 12 of gestation (GD), siRNA was subcutaneously injected into rats (10 mg/kg).On gestational day 14, under isoflurane