<p>Detection of Novel Gene Mutations Associated with Pyrazinamide Resistance in Multidrug-Resistant <em>Mycobacterium tuberculosis</em> Clinical Isolates in Southern China</p>

Abstract

Our study revealed that PZA^R rate in MDR and pre-XDR TB was markedly higher in southern China. The concomitant evaluation of <i>pncA</i> ⁺ UFR, <i>rpsA, panD, Rv2783c,</i> and <i>Rv2044c</i> provides more dependable genotypic results of PZA resistance. Fifty-seven novel mutations/indels in this study may play a vital role as diagnostic markers. The upstream region of <i>pncA</i> and PZase regulation are valuable to explore the unknown mechanism of PZA-resistance.