MiR-370 enhances cell cycle and represses lipid accumulation in porcine adipocytes

Abstract

MicroRNAs (miRNAs) are emerging as an important regulator during adipose development. Previous studies have revealed that miR-370 is related to lipid metabolic homeostasis, however, its roles in lipid accumulation remain to be established. In this study, we demonstrated that ssc-miR-370 was highly conserved across domestic animals, and relatively enriched in adipose tissues and skeletal muscles. Overexpression of ssc-miR-370 significantly promoted the proliferation of porcine preadipocytes through facilitating G₁/S phase transition. Meanwhile, ssc-miR-370 mimics dramatically suppressed adipogenic differentiation, indicated by reduced triglyceride deposition as well as downregulated PPARγ and aP2 expressions. Furthermore, ssc-miR-370 was demonstrated to repress FoxO1 expression via directly targeting FoxO1 3'-UTR using dual luciferase activity assay. Our data evaluates miR-370 as a novel adipogenic modulator, which may be a potential target to reduce backfat thickness in pigs and fight obesity in humans.