Construction of a highly active secretory expression system in Bacillus subtilis of a recombinant amidase by promoter and signal peptide engineering

Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method

Kenneth J. Livak, Thomas D. Schmittgen · Methods · 2001 · 176.9K citations

Functional Genomics, Engineering, Medicine +12

Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar

Gail Lorenz Miller · Analytical Chemistry · 1959 · 28.2K citations

Food Analysis, Altmetric Attention Score, Bibliometrics +17

SignalP 5.0 improves signal peptide predictions using deep neural networks

José Juan Almagro Armenteros, Konstantinos D. Tsirigos, Casper Kaae Sønderby et al. · Nature Biotechnology · 2019 · 4.5K citations · Full text

Signalp 5.0, Engineering, Computational Biology +8

The complete genome sequence of the Gram-positive bacterium Bacillus subtilis

Frank Kunst, Naotaka Ogasawara, Ivan Moszer et al. · Nature · 1997 · 3.7K citations · Full text

TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE

John Spizizen · Proceedings of the National Academy of Sciences · 1958 · 1.8K citations · Full text