Simultaneous Spectrophotometric Estimation of Curcuminoids and Gallic Acid in Bulk Drug and Ayurvedic Polyherbal Tablet Dosage Form

Abstract

In the present study, an attempt has been made to develop an analytical method for the simultaneous estimation of curcuminoids and gallic acid in commercially marketed ayurvedic polyherbal formulation Nisha Amalaki vatti by spectrophotometric method. Simultaneous equations (Vierodt’s method) were performed by UV/Visible spectrophotometric. Curcuminoids has absorbance maxima at about 427nm and Gallic acid maxima at about 227nm in methanol. The linearity was obtained in the concentration range of 10-50 mcg/mL for both curcuminoids and gallic acid. The results were of the analysis were validated statistically and the recovery studies were carried out as per ICH guidelines.