Concepedia
The EMBO Journal · 1995 · 163 citations · 71 references
Open access
Life SciencesCell BiologyMolecular BiologyCell SignalingOncogenesSignal TransductionCell Signaling NetworksProtein Kinase CascadesReceptor Tyrosine Kinase Signaling14-3-3 DomainsErk SignalingExtracellular Signal-regulated Kinase SignalingRaf SignalingRaf-mek-erk Signaling
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Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase
Donald B. Smith, Kevin S. Johnson · Gene · 1988 · 6K citations
Life Sciences, Biochemistry, Molecular Biology +12
The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases
J. Wade Harper · Cell · 1993 · 5.7K citations
Life Sciences, Cell Biology, Molecular Biology +14
Mammalian Ras interacts directly with the serine/threonine kinase raf
Anne B. Vojtek, Stanley M. Hollenberg, Jonathan A. Cooper · Cell · 1993 · 2K citations
Life Sciences, Molecular Biology, Molecular Genetics +14
Eukaryotic proteins expressed in Escherichia coli: An improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase
Kun‐Liang Guan, J.E. Dixon · Analytical Biochemistry · 1991 · 1.8K citations
Life Sciences, Biochemistry, Molecular Biology +16
The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest.
Cheng‐Ting Chien, Paul L. Bartel, Rolf Sternglanz et al. · Proceedings of the National Academy of Sciences · 1991 · 1.5K citations
Life Sciences, Genetics, Molecular Biology +13