Undibacterium piscinae sp. nov., isolated from Korean shiner intestine

Abstract

A novel Gram-stain-negative, non-spore-forming, obligate aerobic, motile, rod-shaped, and flagellated bacterium, designated S11R28^T, was isolated from the intestinal tract of a Korean shiner, <i>Coreoleuciscus splendidus</i>. Based on 16S rRNA gene sequences, strain S11R28^T was identified as member of the genus <i>Undibacterium</i> in class <i>Betaproteobacteria</i>, and was closely related to <i>Undibacterium parvum</i> DSM 23061^T (98.49 %). The isolate grew at 4-25 °C, pH 6-9, with 0 % (w/v) NaCl, and grew optimally at 20 °C, pH 8, in the absence of NaCl. The main cellular fatty acids were C_{16 : 0} and summed features 3 (C_{16 : 1}ω7<i>c</i> and/or C_{16 : 1}ω6<i>c</i>). The strain possessed diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine as predominant polar lipids, and ubiquinone Q-8 as a respiratory quinone. The polyamine profile composed of 2-hydroxyputrescine, spermidine, putrescine, and benzoic acid. A genomic DNA G+C content was 51.4 mol%. The average nucleotide identity between strains S11R28^T and <i>U. parvum</i> DSM 23061^T was 78.66 %. Thus, <i>Undibacterium piscinae</i> can be considered a novel species within the genus <i>Undibacterium</i> with the type strain S11R28^T (=KCTC 62668^T=JCM 33224^T).