SCAR marker for<i>Phytophthora nicotianae</i>and a multiplex PCR assay for simultaneous detection of<i>P. nicotianae</i>and<i>Candidatus</i>Liberibacter asiaticus in citrus

Abstract

A RAPD-SCAR marker-based detection system and the one-step multiplex PCR method developed in this study can be applied to index citrus trees infected (individually or conjointly) with P. nicotianae and CaLas. The present technique developed would also be useful in monitoring disease epidemiology and phytosanitary surveillance.