Engineering Corynebacterium glutamicum to produce the biogasoline isopentenol from plant biomass hydrolysates

Abstract

We describe the successful expression of a heterologous mevalonate-based pathway in the Gram-positive industrial microorganism, <i>C. glutamicum,</i> for the production of the biogasoline candidate, isopentenol. We identified critical genetic factors to harness the isopentenol pathway in <i>C. glutamicum</i>. Further media and cultivation optimization enabled isopentenol production from sorghum biomass hydrolysates.