Direct cloning and heterologous expression of natural product biosynthetic gene clusters by transformation-associated recombination

Yeast transformation: a model system for the study of recombination.

Terry L. Orr‐Weaver, Jack W. Szostak, Rodney Rothstein · Proceedings of the National Academy of Sciences · 1981 · 1.5K citations

Life Sciences, Genetics, Molecular Biology +15

Minimum Information about a Biosynthetic Gene cluster

Marnix H. Medema, Renzo Kottmann, Pelin Yilmaz et al. · Nature Chemical Biology · 2015 · 908 citations · Full text

Life Sciences, Biochemistry, Bioinformatics +11

Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the antibiotic taromycin A

Kazuya Yamanaka, Kirk A. Reynolds, Roland D. Kersten et al. · Proceedings of the National Academy of Sciences · 2014 · 504 citations · Full text

Life Sciences, Biotechnology, Molecular Biology +16

Computational approaches to natural product discovery

Marnix H. Medema, Michael A. Fischbach · Nature Chemical Biology · 2015 · 490 citations · Full text

Pharmacy, Chemistry, Medicinal Chemistry +14

Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting

Jun Fu, Xiaoying Bian, Shengbaio Hu et al. · Nature Biotechnology · 2012 · 447 citations

Life Sciences, Biotechnology, Molecular Biology +12