Preparation of Zinc Oxide Nanoparticles Containing Spray and Barrier Films for Potential Photoprotection on Wound Healing

Abstract

Cutaneous wound healing produces pigmented scars, especially under UV irradiation. In this work, zinc oxide nanoparticles (ZnO NPs) have several morphologies, including nanorods, nanoflowers, and nanospheres. Their size and specific surface area were measured by scanning electron microscopy (SEM) and surface area analysis. The ability of both the ZnO NPs and blends thereof with polymers to protect against phototoxicity was measured using human fibroblast Hs68 cells. Finally, the relative gene expressions of the cellular apoptosis pathway, including p53, Bcl-2, Bax, and Capase 3, were determined by quantitative real-time polymerase chain reaction (qRT-PCR).