Determination of Antioxidant Capacity, Flavonoids and Total Phenolic Content of Extracts from <i>Atractylis flava Desf</i>

Abstract

objective: The aim of the present study was to evaluate the ability of different extracts (PE, DCM, EtAOc and n-BuOH) obtained from Atractylis flava Desf to prevent oxidation using several methods in vitro. Materials and Methods: The capacity of samples for scavenging free radicals (ABTS+, DPPH), preventing lipid peroxidation (s-carotene), chelating metal ions, CUPRAC, reducing power. The quantification of total phenols was performed using Folin-Ciocalteu reagent and the total flavonoid content was performed by the trichloroaluminum method. Results: The highest concentrations of total polyphenols and flavonoids were found in the n-BuOH extract (122,74 ± 0,78 µg GAE/ml and 66,75±6,12 µg QE/ml, respectively). EtAOc and n-BuOH extracts were the most active for scavenging DPPH (IC50 = 158,41±3,24 and IC50 = 427,53±4,69 µg/mL, respectively), and EtAOc extract was also the best for scavenging ABTS+ radicals (IC50 = 15,57±2,93 µg/mL). EtAOc extract possessed the best capacity for preventing lipid peroxidation (IC50 = 16,52±1,87 µg/mL) and CUPRAC assay (A0.50 = 28,58±0,65 µg/mL). The results of ferric reducing power ability of all the tested samples were affected in a dose-dependent manner. Conclusions: These results suggest that Atractylis flava Desf have strong antioxidant potential. Further study is necessary for isolation and characterization of t of the active antioxidant agents, which can be used to treat various oxidative stress-related diseases.