Journal of Lipid Research · 2018 · 43 citations · 57 references
Life SciencesCell BiologyOphthalmologyApoptosisCellular MetabolismOcular DiseaseAge-related Macular DegenerationApoptosis RegulationMacular DegenerationNeurodegeneration MechanismsRetinal DegenerationRetinal Pigment EpitheliumAdvanced Age-related Macular DegenerationLysosomal HydrolasesOxidative Stress InjuryOxidative Stress-induced ApoptosisOxidative Stress-induced Cell DeathRetinal AgingRetinal NeuroprotectionRetinal Pigment Epithelium Cell Death
-acylsphingosine amidohydrolase (ASAH)1] into sphingosine (Sph) is the major source for Sph 1-phosphate production, which has an opposing role to Cer and provides cytoprotection. Here, we investigated the role of Cer in human RPE-derived ARPE19 cells under hydrogen peroxide-induced oxidative stress, and show that Cer and hexosyl-Cer levels increase in the oxidatively stressed ARPE19 cells, which can be prevented by overexpression of lysosomal ASAH1. This study demonstrates that oxidative stress generates sphingolipid death mediators in retinal cells and that induction of ASAH1 could rescue retinal cells from oxidative stress by hydrolyzing excess Cers.
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