Analyzing real-time PCR data by the comparative CT method

Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method

Kenneth J. Livak, Thomas D. Schmittgen · Methods · 2001 · 181.5K citations

Life Sciences, Molecular Biology, Molecular Genetics +9

A new mathematical model for relative quantification in real-time RT-PCR

Michael W. Pfaffl · Nucleic Acids Research · 2001 · 34.9K citations · Full text

Life Sciences, Molecular Biology, Gene Expression +12

Cluster analysis and display of genome-wide expression patterns

Michael B. Eisen, Paul T. Spellman, Patrick O. Brown et al. · Proceedings of the National Academy of Sciences · 1998 · 16.4K citations

Life Sciences, Bioinformatics, Genetics +17

A Multigene Assay to Predict Recurrence of Tamoxifen-Treated, Node-Negative Breast Cancer

Soonmyung Paik, Steven Shak, Gong Tang et al. · New England Journal of Medicine · 2004 · 6.4K citations · Full text

Medicine, Oncology, Breast Oncology +12

Real-time quantification of microRNAs by stem-loop RT-PCR

C. Chen · Nucleic Acids Research · 2005 · 5K citations · Full text

Life Sciences, Biotechnology, Molecular Biology +13