Ultracentrifugation Versus Kit Exosome Isolation: NanoLC–MS and Other Tools Reveal Similar Performance Biomarkers, But Also Contaminations

Abstract

The two isolation methods had an overall similar performance, but we hesitate to use the term 'exosome isolation' as impurities may be present with both isolation methods. NanoLC-MS can detect disease biomarkers in exosomes and is useful for critical assessment of exosome enrichment procedures.