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Evaluation of two-step liquid-liquid extraction protocol for untargeted metabolic profiling of serum samples to achieve broader metabolome coverage by UPLC-Q-TOF-MS

Metabolomic ProfilingSerum SamplesBioanalysisAnalytical ChemistryBiostatisticsBiomarker DiscoveryClinical ChemistryLiquid ChromatographyChromatographyBiochemistryMetabolomicsSample PreparationPharmacologyBroader Metabolome CoverageUntargeted Metabolic ProfilingMass SpectrometryMetabolic ProfilingMetabolismMedicine