Antiadipogenic Effects of Loganic Acid in 3T3-L1 Preadipocytes and Ovariectomized Mice

Abstract

Obesity is caused by an excess storage of body fat, resulting from a chronic imbalance between energy intake and expenditure. <i>Gentiana lutea</i> L. (GL) root has been reported to reduce lipid accumulation in the aortic wall of diabetic rats. Here, we performed fractionation and isolation of the bioactive constituent(s) that may be responsible for the antiadipogenic effects of the GL root extract. A single compound, loganic acid, was identified as a candidate component in the 30% ethanol extract of GL. Loganic acid treatment significantly decreased the adipocyte differentiation of 3T3-L1 preadipocytes in a dose-dependent manner. The expression of key adipogenesis-related genes such as adiponectin (<i>Adipoq</i>), peroxisome proliferator-activated receptor gamma (<i>Pparg</i>), lipoprotein lipase (<i>Lpl</i>), perilipin1 (<i>Plin1</i>), fatty acid binding protein 4 (<i>Fabp4</i>), glucose transporter type 4 (<i>Slc2a4</i>), CCAAT/enhancer-binding protein alpha (<i>Cebpa</i>), and tumor necrosis factor-alpha (<i>Tnf</i>) were significantly reduced following treatment with loganic acid. In vivo experiments in an ovariectomy-induced obesity mouse model showed that loganic acid (oral administration with 10 and 50 mg/kg/day) significantly inhibited body weight gain, total fat increase, fatty hepatocyte deposition in the liver, and adipocyte enlargement in the abdominal visceral fat tissues. These results suggest that loganic acid in the GL root extract has antiadipogenic effects in vitro and in vivo. Loganic acid may be beneficial for the prevention and treatment of obesity, particularly in menopausal obese women.