The diagnostic values of circulating miRNAs for hypertension and bioinformatics analysis

Abstract

Few studies have compared the performances of those reported miRNAs as biomarkers for hypertension in a same cohort, we aimed to comprehensively examine the performances of those reported miRNAs as biomarkers for hypertension and identify the genes and pathways targetted by these miRNAs. Serum samples were collected from patients hospitalized for hypertension in Zhongshan Hospital. Gene expressions of 25 miRNAs were compared between hypertension and normal groups. Receiver operating characteristic (ROC) curves were used to evaluate the accuracy of those miRNAs as biomarkers for hypertension. miRWALK2.0 and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were performed to predict the target genes and pathways of selected miRNAs. A total of 164 participants were enrolled, amongst which 53 were patients with hypertension, 111 were normal population. <i>MiR-122-5p</i> (area under curve (AUC): 0.750), <i>miR-199a-3p</i> (AUC: 0.744), <i>miR-208a-3p</i> (AUC: 0.743), <i>miR-423-5p</i> (AUC: 0.740), and <i>miR-223-5p</i> (AUC: 0.718) showed better performance than others, and the best performance was the combination of <i>miR-199a-3p, miR-208a-3p, miR-122-5p</i>, and <i>miR-223-3p</i> (AUC: 0.80). Pathway analysis revealed that 94 pathways enriched with genes targetted by <i>miR-199a-3p, miR-208a-3p, miR-122-5p, miR-223-5p</i> FoxO signaling was enriched with genes targetted by all the three miRNAs (<i>miR-199a-3p, miR-208a-3p, miR-122-5p</i>). The combination of <i>miR-199a-3p, miR-208a-3p, miR-122-5p</i>, and <i>miR-223-3p</i> has a good diagnostic performance for hypertension, and multitudes of possible mechanisms/pathways through which dysregulation of these miRNAs may impact risk of hypertension.