Journal of Applied Microbiology · 2018 · 15 citations · 33 references
Life SciencesBiotechnologyMicrobiologyBiochemical EngineeringBioreactor DesignMicrobial BiotechnologyBacterial CultivationBacterial FermentationBioprocess OptimizationBioreaction EngineeringMicrobial Enzyme ProductionMicrobial FermentationBioreactor Process DesignExtracellular Enzyme ProductionFermentation Process DesignLipase ProductionLipolytic ActivityLipolytic EnzymesMicrobial Lipase ActivityStatistical Process Optimization
AIM: The aim of this study was to isolate and identify lipolytic bacteria. Perform a statistical stepwise physicochemical optimization for maximum production of extracellular lipase and its validation in a bioreactor. METHODS AND RESULTS: respectively. CONCLUSIONS: The mesophilic and neutrophilic B. stratosphericus PSP8 isolated from petroleum hydrocarbon-contaminated soil is a proper source of lipase. The closeness of the predicted response with that of the experimental value and the enhancement of lipase productivity in fermenter scale by approximately 1·9-fold, showed that statistically optimized design can be used in order to improve the lipase production to meet the increasing demand. SIGNIFICANCE AND IMPACT OF THE STUDY: The RSM-CCF statistical optimization is useful for optimizing a large number of variables and studying their interactive effects on extracellular lipase production.
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Bergey's Manual of Determinative Bacteriology
F. A. S., R. E. Buchanan, N. E. Gibbons et al. · Taxon · 1975 · 11.4K citations