RNA m6A methylation participates in regulation of postnatal development of the mouse cerebellum

Abstract

N 6 -methyladenosine (m 6 A) is an important epitranscriptomic mark with high abundance in the brain. Recently, it has been found to be involved in the regulation of memory formation and mammalian cortical neurogenesis. However, while it is now established that m 6 A methylation occurs in a spatially restricted manner, its functions in specific brain regions still await elucidation. We identify widespread and dynamic RNA m 6 A methylation in the developing mouse cerebellum and further uncover distinct features of continuous and temporal-specific m 6 A methylation across the four postnatal developmental processes. Temporal-specific m 6 A peaks from P7 to P60 exhibit remarkable changes in their distribution patterns along the mRNA transcripts. We also show spatiotemporal-specific expression of m 6 A writers METTL3, METTL14, and WTAP and erasers ALKBH5 and FTO in the mouse cerebellum. Ectopic expression of METTL3 mediated by lentivirus infection leads to disorganized structure of both Purkinje and glial cells. In addition, under hypobaric hypoxia exposure, Alkbh5 -deletion causes abnormal cell proliferation and differentiation in the cerebellum through disturbing the balance of RNA m 6 A methylation in different cell fate determination genes. Notably, nuclear export of the hypermethylated RNAs is enhanced in the cerebellum of Alkbh5 -deficient mice exposed to hypobaric hypoxia. Together, our findings provide strong evidence that RNA m 6 A methylation is controlled in a precise spatiotemporal manner and participates in the regulation of postnatal development of the mouse cerebellum.