Evaluation of PCR primers targeting the<i>groEL</i>gene for the specific detection of<i>Streptococcus agalactiae</i>in the context of aquaculture

Abstract

Due to the importance of S. agalactiae as a pathogen, many PCR primers have been published for this bacterium, designed largely for its detection in dairy and human samples, but many cross-reacting with S. iniae. The ability to differentiate between S. agalactiae and S. iniae in aquaculture derived samples is important as both infect fish, causing similar disease symptoms and are phenotypically similar, yet control strategies and zoonotic risk are species specific.