Analytical Chemistry · 2018 · 40 citations · 31 references
Life SciencesCell BiologyMolecular BiologyBiosensorsMolecular ImagingSignal TransductionCell-based BiosensorsCell-surface ReceptorsFluorescence MicroscopyMolecular Probe DevelopmentMolecular SensorsReceptor ImagingCell Surface Receptor BiosensorsConfocal Fluorescence MicroscopyDisease BiomarkersDna Hybridization AssaysDna Hybridization BiosensorsDna Hybridization DetectionEpidermal Growth Factor Receptor ImagingSignal Amplification Mechanisms
Cell surface receptors play an important role in mediating cell communication and are used as disease biomarkers and therapeutic targets. We present a one-pot molecular toolbox, which we term the split proximity circuit (SPC), for the autonomous detection and visualization of cell surface receptor clusters. Detection was powered by antibody recognition and a series of autonomous DNA hybridization to achieve localized, enzyme-free signal amplification. The system under study was the human epidermal growth factor receptor (HER) family, that is, HER2:HER2 homodimer and HER2:HER3 heterodimer, both in cell lysate and in situ on fixed whole cells. The detection and imaging of receptors were carried out using standard microplate scans and confocal microscopy, respectively. The circuit operated specifically with minimal leakages and successfully captured the receptor expression profiles on three cell types without any intermediate washing steps.
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NIH Image to ImageJ: 25 years of image analysis
Caroline A Schneider, Wayne Rasband, Kevin W. Eliceiri · Nature Methods · 2012 · 65.2K citations