Hydrophilic Multitemplate Molecularly Imprinted Biopolymers Based on a Green Synthesis Strategy for Determination of B-Family Vitamins

Abstract

A novel green synthesis strategy was proposed for preparation of multitemplate molecularly imprinted biopolymers (mt-MIBP) in aqueous media with less consumption of organic solvents, which were subsequently used as sorbents of ultrasound-assisted dispersive solid-phase extraction (d-SPE) for simultaneous recognition and efficient separation of B-family vitamins in juice samples, followed by high performance liquid chromatography (HPLC) determination. The obtained mt-MIBP was fully characterized by SEM, FT-IR, TEM, and BET. It offered high binding capacity, good selectivity, and fast dynamics toward all the templates. Involved parameters in the d-SPE efficiency such as mt-MIBP mass, sonication time, and eluting/washing solvents’ types and volumes were concurrently investigated by central composite design with rapidity and reliability. Under the optimum conditions, the developed mt-MIBP-d-SPE-HPLC method exhibited wide linear range, low limits of detection and quantification (LOQs) within 1.2–5.5 μg L–1 and 4.0–18.4 μg L–1, respectively, and appropriate repeatability (relative standard deviation values below 4.2%, n = 4). The high selectivity of this method makes it suitable for successful monitoring of vitamins in juice samples with satisfactory recoveries of 75.8–92.7%, 81.1–92.5%, and 84.7–93.8% for vitamins riboflavin (B2), nicotinamide (B3), and pyridoxine (B6), respectively. The present study implied highly promising perspectives of water-compatible eco-friendly mt-MIBP for highly effective multiresidue analysis in complicated matrixes.