In vitro Inhibition of Calcium Oxalate Nucleation by Extract-based Fractions of Aerial Parts and Roots of Aerva lanata (Linn.) Juss. ex Schult

Abstract

The current investigation is an attempt to explore the comparative in vitro antiurolithic potential of various phytoconstituent-rich fractions derived from the hydroalcoholic extract of aerial parts and roots of Aerva lanata (Linn.) Juss. ex Schult (Amaranthaceae). The antiurolithic assay was performed by nucleation method. The analysis revealed the phenolic and flavonoid containing fraction from aerial parts to be the most active (71.01±1.13%) amongst the different extract-derived fractions compared to the one obtained from roots (54.61±2.30%). Besides, fluorescence analysis and physicochemical evaluation were carried out according to the official guidelines and supported the quality control of the plant material. Preliminary phytochemical screening of the fractions was also performed and confirmed the presence of various metabolites. Additionally, the content of tannins, total phenolics and flavonoids were also determined spectrophotometrically and found to be comparatively higher in aerial parts (tannin content of 4.34±0.63 mg tannic acid equivalent/g extract, total phenolic content of 127.84±1.50 gallic acid equivalent/g extract and total flavonoid content of 77.61±3.78 rutin equivalent/g extract) than roots (tannin content of 3.03±0.63 mg tannic acid equivalent/g extract, total phenolic content of 98.09±1.10 gallic acid equivalent/ g extract and total flavonoid content of 62.81±5.69 rutin equivalent/g extract). Hence, the present study aids in screening out the best active extract derived fraction exerting litholytic efficacy, which provides a route to further isolation of lead bioactive compounds contributing to significant antiurolithic potency of this traditionally employed herb of immense pharmacological spectrum.