RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination

Abstract

Our results suggest that many other proteins with the PRY/SPRY domain could have yet uncharacterized RNA-binding potential. Together, our data reveal new insights into the molecular roles and characteristics of RNA-binding E3 ubiquitin ligases and demonstrate that RNA could be an essential factor in their enzymatic activity.