Mixed<i> Lactobacillus plantarum</i> Strains Inhibit<i> Staphylococcus aureus</i> Induced Inflammation and Ameliorate Intestinal Microflora in Mice

Abstract

<i>Objective</i>. <i>Staphylococcus aureus</i> is an important pathogen that causes intestinal infection. We examined the immunomodulatory function of single and mixed <i>Lactobacillus plantarum</i> strains, as well as their impacts on the structure of the microbiome in mice infected with <i>Staphylococcus aureus</i>. The experiment was divided into three groups: protection, treatment, and control. Serum IFN-<i>γ</i> and IL-4 levels, as well as intestinal sIgA levels, were measured during and 1 week after infection with <i>Staphylococcus aureus</i> with and without <i>Lactobacillus plantarum</i> treatment. We used 16s rRNA tagged sequencing to analyze microbiome composition. IFN-<i>γ</i>/IL-4 ratio decreased significantly from infection to convalescence, especially in the mixed <i>Lactobacillus plantarum</i> group. In the mixed <i>Lactobacillus plantarum</i> group the secretion of sIgA in the intestine of mice (9.4-9.7 ug/mL) was significantly higher than in the single lactic acid bacteria group. The dominant phyla in mice are <i>Firmicutes</i>, <i>Bacteroidetes</i>, and <i>Proteobacteria</i>. Treatment with mixed lactic acid bacteria increased the anti-inflammatory factor and the secretion of sIgA in the intestine of mice infected with <i>Staphylococcus aureus</i> and inhibited inflammation.