Single-step purification of recombinant Bm86 protein produced in Pichiapastoris by salting-out and by acid precipitation of contaminants

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

Ulrich K. Laemmli · Nature · 1970 · 251.6K citations

Life Sciences, Microbiology, Molecular Biology +14

Commercialisation of a recombinant vaccine against<i>Boophilus microplus</i>

Peter Willadsen, Peter H. Bird, Gary S. Cobon et al. · Parasitology · 1995 · 346 citations

Life Sciences, Biotechnology, Immunology +16

Cloning and expression of a protective antigen from the cattle tick Boophilus microplus.

Keith N. Rand, Thomas C. Moore, Alagacone Sriskantha et al. · Proceedings of the National Academy of Sciences · 1989 · 286 citations · Full text

Life Sciences, Immunology, Molecular Biology +12

High level expression of the B. microplus Bm86 antigen in the yeast Pichia pastoris forming highly immunogenic particles for cattle

Roger Rubiera, Manuel L. Penichet, Raquel Montesinos et al. · Journal of Biotechnology · 1994 · 189 citations

Life Sciences, Immunology, Microbiology +5

Some characteristics of protein precipitation by salts

Y.T. Shih, John M. Prausnitz, Harvey W. Blanch · Biotechnology and Bioengineering · 1992 · 159 citations · Full text

Life Sciences, Biochemistry, Enzymology +12