Journal of Medicinal Chemistry · 2017 · 34 citations · 24 references
MedicineMedicinal ChemistryPharmacologyDrug DesignDrug DiscoveryMolecular PharmacologyBinding KineticsLigand BindingLigand Binding KineticsReceptor BindingReceptor PharmacologyReceptor-ligand InteractionsAzetidine ChemistryBinding ConstantsBioluminescence Resonance Energy TransferFluorescence Resonance Energy TransferFluorescent LabelingFluorescent LabelsFluorescent ProbesKinetic Binding Analysis
The free fatty acid receptor 2 (FFA2/GPR43) is considered a potential target for treatment of metabolic and inflammatory diseases. Here we describe the development of the first fluorescent tracer for FFA2 intended as a tool for assessment of thermodynamic and kinetic binding parameters of unlabeled ligands. Starting with a known azetidine FFA2 antagonist, we used a carboxylic acid moiety known not to be critical for receptor interaction as attachment point for a nitrobenzoxadiazole (NBD) fluorophore. This led to the development of 4 (TUG-1609), a fluorescent tracer for FFA2 with favorable spectroscopic properties and high affinity, as determined by bioluminescence resonance energy transfer (BRET)-based saturation and kinetic binding experiments, as well as a high specific to nonspecific BRET binding signal. A BRET-based competition binding assay with 4 was also established and used to determine binding constants and kinetics of unlabeled ligands.
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Regulation of inflammatory responses by gut microbiota and chemoattractant receptor GPR43
Kendle M. Maslowski, Angélica T. Vieira, Aylwin Ng et al. · Nature · 2009 · 3.2K citations