Fibro/Adipogenic Progenitors (FAPs): Isolation by FACS and Culture

Abstract

Fibro/adipogenic progenitors (FAPs Fibro/adipogenic progenitors (FAPs) ) are tissue-resident mesenchymal stromal cells (MSCs). Current literature supports a role for these cells in the homeostasis and repair of multiple tissues suggesting that FAPs Fibro/adipogenic progenitors (FAPs) may have extensive therapeutic potential in the treatment of numerous diseases. In this context, it is crucial to establish efficient and reproducible procedures to purify FAP Fibro/adipogenic progenitors (FAPs) populations from various tissues. Here, we describe a protocol for the isolation and cell cultureCell culture of FAPs Fibro/adipogenic progenitors (FAPs) from murine skeletal muscle using fluorescenceFluorescence -activated cell sorting (FACS), which is particularly useful for experiments where high cell purity is an essential requirement. Identification, isolation, and cell cultureCell culture of FAPs Fibro/adipogenic progenitors (FAPs) represent powerful tools that will help us to understand the role of these cells in different conditions and facilitate the development of safe and effective new treatments for diseases.