Endogenous Hydrogen Sulfide Homeostasis Is Responsible for the Alleviation of Senescence of Postharvest Daylily Flower via Increasing Antioxidant Capacity and Maintained Energy Status

Abstract

There are limited data concerning the role of endogenous H₂S in prolonging the postharvest of vegetables and fruits. Using a fluorescence microscope with a specific probe, we discovered that, during the senescence of postharvest daylily flower, endogenous H₂S homeostasis was impaired. The activities of two important synthetic enzymes of H₂S, l- and d-cysteine desulfhydrase, exhibited decreasing tendencies. However, NaHS (a H₂S donor) not only blocked the decreased H₂S production but also extended the postharvest life of daylilies. These beneficial roles were verified by the alleviation of lipid peroxidation and the increased activities of antioxidant enzymes. Meanwhile, the energy status was sustained, and the respiration rate was decreased. In contrast to NaHS, the addition of an inhibitor of H₂S synthesis alone aggravated lipid peroxidation and lowered energy charge. Together, the present study implies that endogenous H₂S alleviates senescence of postharvest daylilies via increasing antioxidant capacity and maintained energy status.