Combining independent de novo assemblies optimizes the coding transcriptome for nonconventional model eukaryotic organisms

Abstract

Our approach yields coding transcriptome assemblies that are more likely to be closer to biological reality than any of the three individual assembly packages we investigated. This approach (freely available as a simple perl script) will be of use to researchers working with species for which there is little or no reference data against which the assembly of a transcriptome can be performed.