Application of Strep-Tactin XT for affinity purification of Twin-Strep-tagged CB 2 , a G protein-coupled cannabinoid receptor

The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins

Thomas G.M. Schmidt, Arne Skerra · Nature Protocols · 2007 · 638 citations

Life Sciences, Biochemistry, Molecular Biology +14

The Cannabinoid CB2 Receptor as a Target for Inflammation-Dependent Neurodegeneration

John C. Ashton, Michelle Glass · Current Neuropharmacology · 2007 · 331 citations · Full text

Medicine, Neuroscience, Pharmacology +15

Mutagenesis of a flexible loop in streptavidin leads to higher affinity for the Strep-tag II peptide and improved performance in recombinant protein purification

Stephan D. Voss, Arne Skerra · Protein Engineering Design and Selection · 1997 · 297 citations

Life Sciences, Biochemistry, Molecular Biology +11

The random peptide library-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment

Thomas G.M. Schmidt, Arne Skerra · Protein Engineering Design and Selection · 1993 · 289 citations

Pharmacy, Biochemistry, Biotechnology +17

Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins: uses for protein labeling, detection, and isolation

James D. Hirsch, Leila Eslamizar, Brian Filanoski et al. · Analytical Biochemistry · 2002 · 221 citations

Life Sciences, Biochemistry, Molecular Biology +10