High Performance, Multiplexed Lung Cancer Biomarker Detection on a Plasmonic Gold Chip

Abstract

Diagnosis of lung cancer is performed using a plasmonic gold ( pGOLD ) chip through multiplexed near‐infrared (NIR) detection of carcino‐embryonic antigen (CEA), Cyfra21‐1, and neuron‐specific enolase (NSE) in the serum samples of patients. With ≈50‐fold enhancement of NIR fluorescence, multiplexed microarray analysis of CEA, Cyfra21‐1, and NSE in 10 μL of human serum or whole blood samples on pGOLD chip leads to markedly improved limit‐of‐quantification, limit‐of‐detection, reproducibility, and higher diagnostic sensitivity and specificity compared to traditional biochips and Luminex technology currently in use in hospitals.